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The effect of eight weeks of High Intensity Interval Training (HIIT) on the expression of lipasin in diabetic rats
Poster Presentation
Paper ID : 1124-11THCONF
Authors
1Department of Exercise Physiology, Faculty of Physical Education and sport sciences, Alzahra university, tehran, iran
2Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, Alzahra University, Tehran, Iran
Abstract
Introduction: The prevalence of type 2 diabetes in the world is rising rapidly, due to the proliferation of this disease, insulin resistance, resulting in the destruction of the pancreatic beta cells and eventually disappearing, resulting in Insulin is not secreted. Recent reports of a liver secreted protein have been responsible for liver signaling to beta cells. This protein is the same as betatrophin / lipasin which is called as the expression of the liver in the pancreas and the function of the secreted protein in the pancreas. The aim of this study was to investigate the effect of eight weeks of intensive training (HIIT) on lipasin expression in liver tissue in type 2 diabetic rats.
Methodology: A study was performed on 16 Wistar rats with a mean weight of 160 ± 10 g. After induction of diabetes (feeding rats for seven months with food containing 30% animal fat and 25% fructose), rats They were randomly divided into two groups control and HIIT. The training program was run for 8 weeks and 5 sessions per week on the treadmill running. Exercise with two periods (two-minute) of intense activity (80-90% of maximum oxygen intake) in the first week, with four replicates in the fourth week and at the end of the eighth week; between any two high-intensity alternatives, two minutes of low intensity regression (30% Maximum Oxygen intake). Twenty-four hours after the last training session, the animals were sacrificed and the lipasin gene expression from liver tissue was measured using qPCR Real Time method. Independent t-test was used for data analysis and Pearson correlation was used to determine the correlation between lipasin expression and insulin resistance index at P < 0.05.
Results: The results showed that after eight weeks of training, the expression of the lipasin gene expression in the liver of the rats in the training group was significantly higher than the control group (P = 0.037); also, a significant negative correlation was observed between the expression of the lipasin gene and the insulin resistance index in the training group was compared to the control group (r = -0.560, P = 0.037).
Discussion: It seems that the implementation of the eight-week HIIT, by increasing the expression of the lipasin gene, can increase the beta cells in diabetic patients, and may be an effective non-pharmacologic intervention to reduce the symptoms and complications of the disease.
Methodology: A study was performed on 16 Wistar rats with a mean weight of 160 ± 10 g. After induction of diabetes (feeding rats for seven months with food containing 30% animal fat and 25% fructose), rats They were randomly divided into two groups control and HIIT. The training program was run for 8 weeks and 5 sessions per week on the treadmill running. Exercise with two periods (two-minute) of intense activity (80-90% of maximum oxygen intake) in the first week, with four replicates in the fourth week and at the end of the eighth week; between any two high-intensity alternatives, two minutes of low intensity regression (30% Maximum Oxygen intake). Twenty-four hours after the last training session, the animals were sacrificed and the lipasin gene expression from liver tissue was measured using qPCR Real Time method. Independent t-test was used for data analysis and Pearson correlation was used to determine the correlation between lipasin expression and insulin resistance index at P < 0.05.
Results: The results showed that after eight weeks of training, the expression of the lipasin gene expression in the liver of the rats in the training group was significantly higher than the control group (P = 0.037); also, a significant negative correlation was observed between the expression of the lipasin gene and the insulin resistance index in the training group was compared to the control group (r = -0.560, P = 0.037).
Discussion: It seems that the implementation of the eight-week HIIT, by increasing the expression of the lipasin gene, can increase the beta cells in diabetic patients, and may be an effective non-pharmacologic intervention to reduce the symptoms and complications of the disease.
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