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The effects of saffron supplementation on the relation between HSP and cytokines following strenuous physical activity in male mountain climbers
Poster Presentation
Paper ID : 1222-11THCONF
Authors
1Fatemeh Jan Bozorgi 
; 2Farshad Ghazalian; 3Negar Borghei; 4Parmida Hajikhani; 5Mina Naderali; 5Mahshid Dejan; 1Fatemeh Saebi
; 2Farshad Ghazalian; 3Negar Borghei; 4Parmida Hajikhani; 5Mina Naderali; 5Mahshid Dejan; 1Fatemeh Saebi
1phd student Department of Physical Education, Science and Research Branch, Islamic Azad University, Tehran, Iran
2Assistant Professor Department of physical education science and research branch islamic azad uinversity tehran iran
3Master of exercise Physiology ,Science and Research Branch, Islamic Azad University, Tehran, Iran
4phd student Department of Physical Education, Tehran University, Tehran, Iran
5Master of exercise Physiology, Science and Research Branch, Islamic Azad University, Tehran, Iran
Abstract
Introduction: The aim of this study was to evaluate the relation between Heat Shock Protein 70 (HSP-70), Interleukin-6 (IL-6) and Tumor Necrosis Factor-α (TNF-α) in one bout of strenuous physical activity with and without saffron supplementation in male mountain climbers.
Methodology: Participants were eleven male mountain climbers with 27-37 years of age. The physical activity session included running on a treadmill with %-5 gradient for 45 minutes with %80-90 maximum heart rate. Blood samples were taken in three different times- before, immediately and one hour after the physical activity- in order to measure HSP-70, IL-6 and TNF-α. After three days of recovery, participants started consuming 300 mg/d capsulated dried saffron stigma for 10 days. The physical activity session was repeated and blood samples were again taken before, immediately and one hour after it. Pearson's chi-squared test was used for analysis of results (α= 0.05).
Results: The physical activity protocol resulted in an increase in studied pro-inflammatory cytokines and HSP70 both before and after supplementation. Before the first physical activity session and supplementation, positive and considerable correlation was shown between IL-6 and TNF-α. Despite the relationships in other stages, none of them were statistically considerable.
Before consumption of saffron capsules, TNF-α and IL-6 had negative considerable correlations immediately and one hour after physical activity. Both IL-6 and TNF-α decreased after one hour of physical activity, but IL-6 decreased more than TNF-α and they had contrary concentrations, meaning the more IL-6 decreased, TNF-α showed less decrease. Further results show that TNF-α and HSP70 decreased immediately and one hour after physical activity both before and after supplementation and their trends were positively and significantly correlated.
Discussion:Ten days Saffron supplementation showed positive influences on mentioned immune responses after physical activity. Due to supplement’s antioxidant and anti-inflammatory ingredients,a positive correlation between TNF-α and HSP-70 happened immediately and one hour after physical activity.
Methodology: Participants were eleven male mountain climbers with 27-37 years of age. The physical activity session included running on a treadmill with %-5 gradient for 45 minutes with %80-90 maximum heart rate. Blood samples were taken in three different times- before, immediately and one hour after the physical activity- in order to measure HSP-70, IL-6 and TNF-α. After three days of recovery, participants started consuming 300 mg/d capsulated dried saffron stigma for 10 days. The physical activity session was repeated and blood samples were again taken before, immediately and one hour after it. Pearson's chi-squared test was used for analysis of results (α= 0.05).
Results: The physical activity protocol resulted in an increase in studied pro-inflammatory cytokines and HSP70 both before and after supplementation. Before the first physical activity session and supplementation, positive and considerable correlation was shown between IL-6 and TNF-α. Despite the relationships in other stages, none of them were statistically considerable.
Before consumption of saffron capsules, TNF-α and IL-6 had negative considerable correlations immediately and one hour after physical activity. Both IL-6 and TNF-α decreased after one hour of physical activity, but IL-6 decreased more than TNF-α and they had contrary concentrations, meaning the more IL-6 decreased, TNF-α showed less decrease. Further results show that TNF-α and HSP70 decreased immediately and one hour after physical activity both before and after supplementation and their trends were positively and significantly correlated.
Discussion:Ten days Saffron supplementation showed positive influences on mentioned immune responses after physical activity. Due to supplement’s antioxidant and anti-inflammatory ingredients,a positive correlation between TNF-α and HSP-70 happened immediately and one hour after physical activity.
Keywords
Saffron, Heat Shock Protein, Interleukin-6, Tumor Necrosis Factor-α , Strenuous Physical Activity
Subjects