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The effect of high intensity interval training on the gene expression of nicotinic AchR in gastrocnemius muscle of male rats
Poster Presentation
Paper ID : 1234-11THCONF
Authors
1Associate professor, University of Zanjan
2دانشجوی کارشناسی ارشد
3استادیار دانشگاه زنجان
4کارشناسی ارشد
Abstract
Introduction: Nicotinic Acetylcholine Receptors (nAchR) are the integral membrane proteins and prototypical members of the ligand-gated ion-channel super family that modify synaptic transmission at the neuromuscular junction. Previous studies have shown that endurance and resistance training improves AchRs in neuromuscular junction. Therefore, the purpose of the present study was to the assessment of the effect of high intensity interval training on the gene expression of nicotinic AchR in gastrocnemius muscle of Male Wistar rats.
Methodology: In this experimental study, 12 male Wistar rats (mean weight = 289.95±11.295 gr), after one week familiarization with environmental conditions and training, were randomly divided into two groups (control and HIIT) and they carried out the training protocol for 8 weeks (10 times for 1 min running on treadmill with the speed of 30-45 m/min and 2 min rest interval at the first week which increased to the 70-85 m/min and 3 min rest intervals at the last weeks). An under loading week was taken at week 5 to prevent from overtraining. Forty-eight hours after the last session of training, animals were anaesthetized and their gastrocnemius muscles excised. For assessment of the gene expression of nicotinic AchR, REAL TIME PCR method was used. Independent- sample t-test in SPSS 22.0 was used to analyze the data.
Results: Data analysis showed that the gene expression of nicotinic AchR in gastrocnemius muscle was significantly (P=0.000) higher in HIIT group (9.91±0.49) than control groups (3.49±0.32). Therefore, HIIT increases the gene expression of nAcR in gastrocnemius muscle.
Discussion: The gene expression of nAchR increased with HIIT protocol in gastrocnemius muscles. It seems that high intensity interval training can lead to accelerating synaptic transcription factors and clustering of nAchR that contribute to the facilitation of synaptic transmission. So, training with low volume but high intensity could improve neuromuscular junction's transmission as useful as endurance training.
Methodology: In this experimental study, 12 male Wistar rats (mean weight = 289.95±11.295 gr), after one week familiarization with environmental conditions and training, were randomly divided into two groups (control and HIIT) and they carried out the training protocol for 8 weeks (10 times for 1 min running on treadmill with the speed of 30-45 m/min and 2 min rest interval at the first week which increased to the 70-85 m/min and 3 min rest intervals at the last weeks). An under loading week was taken at week 5 to prevent from overtraining. Forty-eight hours after the last session of training, animals were anaesthetized and their gastrocnemius muscles excised. For assessment of the gene expression of nicotinic AchR, REAL TIME PCR method was used. Independent- sample t-test in SPSS 22.0 was used to analyze the data.
Results: Data analysis showed that the gene expression of nicotinic AchR in gastrocnemius muscle was significantly (P=0.000) higher in HIIT group (9.91±0.49) than control groups (3.49±0.32). Therefore, HIIT increases the gene expression of nAcR in gastrocnemius muscle.
Discussion: The gene expression of nAchR increased with HIIT protocol in gastrocnemius muscles. It seems that high intensity interval training can lead to accelerating synaptic transcription factors and clustering of nAchR that contribute to the facilitation of synaptic transmission. So, training with low volume but high intensity could improve neuromuscular junction's transmission as useful as endurance training.
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