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Investigate the effects of high intensity interval training (HIIT) and moderate-intensity continuous training (MICT) on VEGF gene expression in visceral and subcutaneous adipose tissues in male Wistar rats
Poster Presentation
Paper ID : 1262-11THCONF
Authors
1Assistant professor, Department of Physical Education and Sport Sciences, Faculty of Humanities, Shahrekord University, Shahrekord, Iran.
2Graduated student of Exercise Physiology, University of Payam Noor, Iran
3Associate professor, Exercise Physiology, University of Payam Noor, Iran
Abstract
Introduction: The purpose of present study was to investigate the effects of high intensity interval training ( HIIT ) and moderate-intensity continuous training (MICT) on VEGF gene expression in visceral and subcutaneous adipose tissues in male Wistar rats.
Methodology: In this experimental study, 24 male Wistar rats with 250±20 gram body masses divided to basal control (n=6), 8 weeks control (n=6), MICT (n=6), and HIIT (n=6) groups as study sample. Training program consists of 8 weeks and 5 days per weeks running on treadmill. The intensity of MICT protocol was 50-75% of VO2 max (15 to 30 meters per minute), and it`s duration was about 15 to 60 minutes. The HIIT protocol was 4-8×1 min with 70-100 % of VO2 max (28 to 55 meters per minute) and 1 min recovery with 50-70 % VO2 max (12 to 30 meters per minute) and total duration was 15 to 23 minutes. Adipose tissue sample were removed 48 hours after last training session and VEGF gene expression measured by Real-Time PCR methods. Statistical significant levels set at p≤0.05.
Results: Results indicated that both MICT and HIIT induces significant increase in subcutaneous VEGF gene expression (P≤0.05), whereas, no significant changes in visceral VEGF gene expression happened after both training protocol, neither between groups changes observed in subcutaneous and visceral adipose tissue VEGF gene expression (P≥0.05).
Discussion: Occurring to the results it seems that the extent of exercise training effects on adipose tissue angiogenesis is more depend on exercise intensity rather than to exercise duration.
Methodology: In this experimental study, 24 male Wistar rats with 250±20 gram body masses divided to basal control (n=6), 8 weeks control (n=6), MICT (n=6), and HIIT (n=6) groups as study sample. Training program consists of 8 weeks and 5 days per weeks running on treadmill. The intensity of MICT protocol was 50-75% of VO2 max (15 to 30 meters per minute), and it`s duration was about 15 to 60 minutes. The HIIT protocol was 4-8×1 min with 70-100 % of VO2 max (28 to 55 meters per minute) and 1 min recovery with 50-70 % VO2 max (12 to 30 meters per minute) and total duration was 15 to 23 minutes. Adipose tissue sample were removed 48 hours after last training session and VEGF gene expression measured by Real-Time PCR methods. Statistical significant levels set at p≤0.05.
Results: Results indicated that both MICT and HIIT induces significant increase in subcutaneous VEGF gene expression (P≤0.05), whereas, no significant changes in visceral VEGF gene expression happened after both training protocol, neither between groups changes observed in subcutaneous and visceral adipose tissue VEGF gene expression (P≥0.05).
Discussion: Occurring to the results it seems that the extent of exercise training effects on adipose tissue angiogenesis is more depend on exercise intensity rather than to exercise duration.
Keywords
Adipose tissue; Angiogenesis; Continues aerobic exercise; High Intensity Interval Training (HIIT); Vascular endothelial growth factor
Subjects