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Effect of timing of whey protein supplement on creatine kinase (CK) and lactate dehydrogenase (LDH) after eccentric exercise in healthy women
Poster Presentation
Paper ID : 1281-11THCONF
Authors
1university ardakan
2ardakan universitiy
3ardakan university
Abstract
Introduction: It is well known that repeated high-force eccentric exercise induces muscle damage. Muscle damage reduces maximal isometric strength and increases muscle soreness and blood markers such as creatine kinase (CK) and lactate dehydrogenase (LDH). Whey protein is commonly ingested after exercise, and it has been used as a supplement to prevent muscle degeneration and to increase protein synthesis. However, the timing when whey protein supplement must be ingested after muscle-damaging exercise is debatable. Therefore, the present study investigated the effects of the timing of supplement ingestion on muscle damage markers after eccentric exercise.
Methodology: For this purpose, 60 women participated in this study; they were randomly assigned to control group (28.17±4.61 years; 163.10±5.57 cm; 74.42±7.42 kg; n=20), before supplement group (27.79±3.51 years; 162.16±7.16 cm; 72.30±4.57 kg; n=20), after supplement group (26.37±6.28 years; 167.42±3.80 cm; 71.69±5.60 kg; n=20), or before and after supplement group (29.21±3.04 years; 170.15±3.33 cm; 71.87±5.60 kg; n=20). Eccentric exercise was performed using Knee flexors with a modified preacher curl machine. Subsequently, maximal isometric strength, muscle soreness and blood markers (creatine kinase (CK) and lactate dehydrogenase (LDH)) were measured before and after exercise under similar conditions and using standard laboratory tools. Repeated-measure analysis of variance was used to analyze the effects of timing of supplement. Significance was set at 0.05.
Results: Maximal isometric strength were significantly reduced following eccentric exercise (P=0.021), while muscle soreness and blood markers were significantly increased after exercise (P=0.015). However, there were no significant effects of the timing of supplement ingestion on maximal isometric strength (P=0.516), but there were significant effects of the timing of supplement ingestion on muscle soreness (P=0.037), CK (P=0.025) and LDH (P=0.031) following eccentric exercise.
Discussion: Thus, based on the results of this study the timing of whey protein supplement did affect reduction of muscle damage or recovery following eccentric exercise.
Methodology: For this purpose, 60 women participated in this study; they were randomly assigned to control group (28.17±4.61 years; 163.10±5.57 cm; 74.42±7.42 kg; n=20), before supplement group (27.79±3.51 years; 162.16±7.16 cm; 72.30±4.57 kg; n=20), after supplement group (26.37±6.28 years; 167.42±3.80 cm; 71.69±5.60 kg; n=20), or before and after supplement group (29.21±3.04 years; 170.15±3.33 cm; 71.87±5.60 kg; n=20). Eccentric exercise was performed using Knee flexors with a modified preacher curl machine. Subsequently, maximal isometric strength, muscle soreness and blood markers (creatine kinase (CK) and lactate dehydrogenase (LDH)) were measured before and after exercise under similar conditions and using standard laboratory tools. Repeated-measure analysis of variance was used to analyze the effects of timing of supplement. Significance was set at 0.05.
Results: Maximal isometric strength were significantly reduced following eccentric exercise (P=0.021), while muscle soreness and blood markers were significantly increased after exercise (P=0.015). However, there were no significant effects of the timing of supplement ingestion on maximal isometric strength (P=0.516), but there were significant effects of the timing of supplement ingestion on muscle soreness (P=0.037), CK (P=0.025) and LDH (P=0.031) following eccentric exercise.
Discussion: Thus, based on the results of this study the timing of whey protein supplement did affect reduction of muscle damage or recovery following eccentric exercise.
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