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The effect of high intensity interval training and moderate-intensity continuous training on MEF2A and MEF2D gene expression in skeletal muscle of obese male rats
Oral Presentation
Paper ID : 1505-11THCONF
Authors
1Ph.D. student of exercise Physiology, Islamic Azad University, Science and Research Branch, Tehran
2Associate professor, Exercise Physiology, University of Payam Noor, Iran
3Assistant professor, Department of Physical Education and Sport Sciences, Faculty of Humanities, Shahrekord University, Shahrekord, Iran.
Abstract
Introduction: The Myocyte Enhancer Factors (MEFs) are kinds of proteins that facilitate the GLUT4 function in different tissues. The aim of study was to investigate the effect of 8 weeks of high intensity interval training (HIIT) and moderate-intensity continuous training (MICT) on the MEF2A and MEF2D gene expression in skeletal muscle of obese male rats.
Methodology: Twenty eight male Wistar rats with 150 25 grams body weight divided to base control (n = 6), obese control (n = 6), HIIT (n = 8), and MICT (n = 8) groups as study sample. At the beginning of the study, base control group rats were sacrificed. In other groups, rats underwent high fat regime diet for 8 weeks. Training program consists of 8 weeks and 5 days per weeks running on treadmill. In MICT group, training protocol was consist of 25 to 60 minutes continuous running with 15 to 22 meters per minute. The HIIT protocol was consist 5-12×15-30 seconds intermittent running with 29-36 meter per min and 1 min active recovery between high intervals. Forty-eight hour after the last session, the rats were scarified and gastrocnemius muscle tissue sample were removed. MEF2A and MEF2D gene expression measured by Real-Time PCR methods. The one-way ANOVA and independent sample t-test used for statistical analysis (P ≤ 0.005).
Results: Results indicated that neither MICT nor HIIT changed MEF2A gene expression in gastrocnemius muscle of obese rats (P = 0.068). But HIIT training significantly increased MEF2D gene expression in this tissue (P = 0.042).
Discussion: According to the results, the HIIT training for 8 weeks, increased gene expression of MEF2D in muscle fibers in obese rats. Therefore, glucose uptake probably improve by increase of MEF2D and subsequently GLUT4 increase.
Methodology: Twenty eight male Wistar rats with 150 25 grams body weight divided to base control (n = 6), obese control (n = 6), HIIT (n = 8), and MICT (n = 8) groups as study sample. At the beginning of the study, base control group rats were sacrificed. In other groups, rats underwent high fat regime diet for 8 weeks. Training program consists of 8 weeks and 5 days per weeks running on treadmill. In MICT group, training protocol was consist of 25 to 60 minutes continuous running with 15 to 22 meters per minute. The HIIT protocol was consist 5-12×15-30 seconds intermittent running with 29-36 meter per min and 1 min active recovery between high intervals. Forty-eight hour after the last session, the rats were scarified and gastrocnemius muscle tissue sample were removed. MEF2A and MEF2D gene expression measured by Real-Time PCR methods. The one-way ANOVA and independent sample t-test used for statistical analysis (P ≤ 0.005).
Results: Results indicated that neither MICT nor HIIT changed MEF2A gene expression in gastrocnemius muscle of obese rats (P = 0.068). But HIIT training significantly increased MEF2D gene expression in this tissue (P = 0.042).
Discussion: According to the results, the HIIT training for 8 weeks, increased gene expression of MEF2D in muscle fibers in obese rats. Therefore, glucose uptake probably improve by increase of MEF2D and subsequently GLUT4 increase.
Keywords
Myocyte enhancer factor; Moderate intensity continuous training; High intensity interval training; Skeletal Muscle; Obese rats
Subjects