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The effect of saffron consumption and intensive exercise on serum levels of Interleukin-6 (IL-6), Tumor Necrosis Factor Alpha (TNF-α) and cortisol in young men
Poster Presentation
Paper ID : 1551-11THCONF
Authors
1University of Birjand
2Birjand University - Department of Physical Education and Sport Sciences
3irainan
Abstract
Introduction: The aim of this study was to investigate the effect of 15 days of taking saffron extract with an intensive exercise session on serum levels of interleukin-6, Tumor Necrosis Factor and cortisol in young men with a maximum heart rate of 85%.
Methodology: For this purpose, 24 male students were volunteered and divided into two equal groups (saffron and placebo) based on their aerobic capacity. All subjects were taken 7 milliliters of blood at rest. After sampling for 30 minutes, 85% of the heart rate was stored on a treadmill without incline and immediately after the completion of the activity they again bleed. After completion of the pre-test for 15 days, the two groups were completed, so that the saffron group received 400 mg of saffron extract per day and strch in the placebo group. After completion of supplements, such as pre-test, two blood samples were taken before and after 30 minutes of the subjects. Serum levels of IL-6 and TNF-α were determined by ELISA and serum cortisol levels using radioimmunoassay method. Statistical analysis of the data was carried out through ANCOVA test at a significance level of p≤0.05.
Results: The results showed that after the supplement the blood cortisol concentration of the rest and after the activity decreased significantly (P=0.04, P=0.01). However, there was no significant effect on IL-6 concentration (p≥0.05). There was no significant difference in resting TNF-α (p≥0.05), but its level was significantly decreased after activity (p=.02).
Discussion: According to the results of this study, saffron supplements can restore inflammatory indices and levels of stress-induced hormone-induced stress from baseline levels and be useful to athletes involved in extreme sports activities.
Methodology: For this purpose, 24 male students were volunteered and divided into two equal groups (saffron and placebo) based on their aerobic capacity. All subjects were taken 7 milliliters of blood at rest. After sampling for 30 minutes, 85% of the heart rate was stored on a treadmill without incline and immediately after the completion of the activity they again bleed. After completion of the pre-test for 15 days, the two groups were completed, so that the saffron group received 400 mg of saffron extract per day and strch in the placebo group. After completion of supplements, such as pre-test, two blood samples were taken before and after 30 minutes of the subjects. Serum levels of IL-6 and TNF-α were determined by ELISA and serum cortisol levels using radioimmunoassay method. Statistical analysis of the data was carried out through ANCOVA test at a significance level of p≤0.05.
Results: The results showed that after the supplement the blood cortisol concentration of the rest and after the activity decreased significantly (P=0.04, P=0.01). However, there was no significant effect on IL-6 concentration (p≥0.05). There was no significant difference in resting TNF-α (p≥0.05), but its level was significantly decreased after activity (p=.02).
Discussion: According to the results of this study, saffron supplements can restore inflammatory indices and levels of stress-induced hormone-induced stress from baseline levels and be useful to athletes involved in extreme sports activities.
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