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The effect of eight-week high intensity interval training (HIIT) on FNDC5 gene expression in type 2 diabetic rats
Poster Presentation
Paper ID : 1561-11THCONF
Authors
1Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, Alzahra University, Tehran, Iran
2Department of Exercise Physiology, Faculty of Physical Education and sport sciences, Alzahra university, tehran, iran
Abstract
Introduction: Metabolic disorders in skeletal musclesis one of the most important factors in the development of type 2 diabetes which includes many diabetic cases. The skeletal muscle secretes a variety of signaling molecules called myokine. A new myokine is a proteolytic irisin derived from FNDC5. A new theory is on changing the phenotype of fatty tissue through training which is probably developed as a result of muscle FNDC5 gene expression. FNDC5 is released as irisin in the bloodafter separating from the membrane and decomposing, and after reaching the target cell it leads to increase in UCP1 expression leveland ultimately white fat turns into brown fat. Fatty tissue browning is beneficial for treatment of many metabolic diseases such as obesity, insulin resistance. The purpose of this study was to investigate the effect of eight-week High-intensity interval training (HIIT) on muscle FNDC5 gene expression in type 2 diabetic rats.
Methodology: For this purpose, 16 Wistar rats with an average weight of 160 ± 10 grams were randomly assigned into two groups of eight, after induction of diabetes (seven months of feeding rats with high-fat and sweet foods): control, HIIT. The control group did not have any exercise activity. The HIIT group performed their sport protocol with 90% of maximum speed (five days per week for eight weeks). 24 hours after the last exercise session and one simple food at night they were anesthetized with intraperitoneal injection of ketamine and xylazine. Then blood samples were collected directly from their hearts and serum separation was performed. Their muscle tissue (soleus) was isolated under sterile conditions and after being homogenized was kept at -80 °C. RT-PCR was used to measure the expression level of FNDC5 gene. The statistical test of one way ANOVA was used to determine the significance of the differences between the research groups. The significance level for all analyses is P≤0.05.
Results: The relative mRNA expression level of FNDC5 gene has increased significantly after HIIT.
Discussion: Although the study on this theory still needs more attempts, the current resultsillustrate that HIIT can improve type 2 diabetes byincreasing the expression level of muscle FNDC5 gene.
Methodology: For this purpose, 16 Wistar rats with an average weight of 160 ± 10 grams were randomly assigned into two groups of eight, after induction of diabetes (seven months of feeding rats with high-fat and sweet foods): control, HIIT. The control group did not have any exercise activity. The HIIT group performed their sport protocol with 90% of maximum speed (five days per week for eight weeks). 24 hours after the last exercise session and one simple food at night they were anesthetized with intraperitoneal injection of ketamine and xylazine. Then blood samples were collected directly from their hearts and serum separation was performed. Their muscle tissue (soleus) was isolated under sterile conditions and after being homogenized was kept at -80 °C. RT-PCR was used to measure the expression level of FNDC5 gene. The statistical test of one way ANOVA was used to determine the significance of the differences between the research groups. The significance level for all analyses is P≤0.05.
Results: The relative mRNA expression level of FNDC5 gene has increased significantly after HIIT.
Discussion: Although the study on this theory still needs more attempts, the current resultsillustrate that HIIT can improve type 2 diabetes byincreasing the expression level of muscle FNDC5 gene.
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