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The effect of synbiotic supplementation on hemoglobin and proliferation and antioxidant capacity of red blood cells after exhaustive activity in inactive young women
Poster Presentation
Paper ID : 1563-11THCONF
Authors
1Department of Sport Physiology university of Gilan
2Department of Biology, University of Guilan
Abstract
Introduction: Recent findings suggest that probiotics can increase the absorption of minerals such as iron from the intestines and indeed have proven ability to increase the antioxidant defense of the body. In this study, the effect of synbiotic supplementation on hemoglobin and proliferation and antioxidant capacity of red blood cells after exhaustive activity in passive girls was evaluated.
Methodology: This experimental study was done blindly on 18 subjects (complementary group: 9 and placebo group: 9) non-athlete girl with an average age of 25.72±0.83 years, height 166.34±5.35 cm, 62.18±6.8 kg, body mass index was 23.7±1.33 kg/m2 and the maximum oxygen power was 32.22±1.85 kg/kg/min. Subjects received 2 daily synbiotic capsules (one plus breakfast plus one dinner) for 6 weeks each day. Placebo group received starch .Subjects performed the exhausting activity (Bruce test) in the fasting phase of the lethal phase. Blood samples were collected in two stages before and after the end of 6 weeks of supplementation and after exercise. Hemoglobin levels and red blood cell proliferation were measured by CBC assay and oxidative stress factors (superoxide dismutase, glutathione, hydrogen peroxide, and total antioxidant capacity) through analytical kits. Data analysis was performed using 2×2 factor analysis of variance was observed (P<0.05).
Results: There was no significant difference in superoxide dismutase and glutathione erythrocyte enzymes in the supplemented and placebo groups. There was a significant increase in total antioxidant capacity of synbiotic supplement group (P<0.05). There was no change in the number of RBCs and hemoglobin increased after 6 weeks of synbiotic supplementation.
Discussion: It seems that the use of 6 days of synbiotic supplementation did not increase the antioxidant enzymes, the number of red blood cells, the hemoglobin content and the reduction of hydrogen peroxide, but significantly increased the total antioxidant capacity of the body.
Methodology: This experimental study was done blindly on 18 subjects (complementary group: 9 and placebo group: 9) non-athlete girl with an average age of 25.72±0.83 years, height 166.34±5.35 cm, 62.18±6.8 kg, body mass index was 23.7±1.33 kg/m2 and the maximum oxygen power was 32.22±1.85 kg/kg/min. Subjects received 2 daily synbiotic capsules (one plus breakfast plus one dinner) for 6 weeks each day. Placebo group received starch .Subjects performed the exhausting activity (Bruce test) in the fasting phase of the lethal phase. Blood samples were collected in two stages before and after the end of 6 weeks of supplementation and after exercise. Hemoglobin levels and red blood cell proliferation were measured by CBC assay and oxidative stress factors (superoxide dismutase, glutathione, hydrogen peroxide, and total antioxidant capacity) through analytical kits. Data analysis was performed using 2×2 factor analysis of variance was observed (P<0.05).
Results: There was no significant difference in superoxide dismutase and glutathione erythrocyte enzymes in the supplemented and placebo groups. There was a significant increase in total antioxidant capacity of synbiotic supplement group (P<0.05). There was no change in the number of RBCs and hemoglobin increased after 6 weeks of synbiotic supplementation.
Discussion: It seems that the use of 6 days of synbiotic supplementation did not increase the antioxidant enzymes, the number of red blood cells, the hemoglobin content and the reduction of hydrogen peroxide, but significantly increased the total antioxidant capacity of the body.
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