The effect of endurance training on the expression of UCP2 mRNA in visceral brown adipose tissue of obese male C57BL/6 mice
Oral Presentation XML
Paper ID : 1609-11THCONF
1دانشگاه اصفهان/دانشکده تربیت بدنی و علوم ورزشی
2استاد دانشگاه اصفهان
3دانشگاه اصفهان/ دانشکده زیست شناسی
4پژوهشگاه رویان
Introduction: The mitochondrial uncoupling proteins (UCPs) plays an important role in energy balance. The main outcome of UCP functions is decrease in ATP production resulting in regulation of fuel metabolism and reduction in reactive oxygen species (ROS) production. Additionally, Ucp2 interfere in free fatty acids (FFA) oxidation and glucose tolerance; which ultimately prevents progress of obesity and T2D. This mRNA disappears the electrochemical gradient in electron transport chain and uncouple the respiration with releasing heat. Recent studies demonstrated that physical activity causes an increase in Ucp2 content. Also elevated stimulation of FFA as one of the main stimulation of Ucp2 mRNA is observed during high fat diet (HFD). Therefore; the aim of present study is to determine the effect of endurance training and HFD on the expression of Ucp2 mRNA in visceral white adipose tissue (visWAT) of male C57BL/6 mice.
Methodology: 24 male mice fed HFD which was consist of 60% fat and exercised whit moderate intensity on treadmill, during 8 weeks in four group as (HF-EX) (HF- SED) (LF-EX) (LF- SED) which was divided randomly. 24 hours after the last practice session they sacrificed and their visWAT isolated for gene expression with real time PCR. Two-way ANOVA and significance level were considered as p˂0.05.
Results: weight gaining and calories consumption of mice were significantly correlated (p˂0.05). Result showed a significant increase in Ucp2 level in visWAT of trained group due to 8 week endurance training and HFD (p˂0.05).
Discussion: It was shown that Ucp2 expression elevated due to HFD and endurance training. Elevation of ROS all during endurance training and HFD is one of the major reason of this elevation. Although with the elevation of this marker in adipose tissue, which is one of the important metabolic tissues, illustrate the importance of Ucp2 mRNA and its substantial role in prevention of obesity.