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Sex-dependent responses in rat heart to short and long high intensity interval exercise bouts: Telomerase activity, total oxidant/antioxidant status
Poster Presentation
Paper ID : 1636-11THCONF
Authors
1Department of Exercise Physiology, Faculty of Exercise Physiology, University of Birjand
2Department of Exercise Physiology, Faculty of Exercise Physiology, University of Birjand, Birjand, Iran
3Cellular and Molecular Endocrine Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences
4Ph.D student at Islamic Azad University, Science and Research Branch, Tehran, Iran
Abstract
Introduction: Cardiovascular diseases is prevalence increases with age that varies according to gender. Telomerase and/or telomeres play a role in aging, aging-related conditions and disease, and life span. Exercise training was known as a way of preventing and delaying many age-related diseases. Despite described benefits of high-intensity interval exercise (HIIE), the acute responses following HIIE with different time of bouts and associated health risks have only been sparsely discovered in different gender. The aim of this study was to investigate the sex-dependent responses to short and long high intensity interval exercise bouts on telomerase enzyme activity, total oxidant status (TOS) and total antioxidant capacity (TAC) in heart muscle of healthy male and female rats.
Methodology: 40 male Wistar rats (aged 12 weeks) were randomly assigned to six groups: male and female control (two groups), male and female short bouts HIIE (MHIIESh and FMHIIESh respectively, 16×1 min work at of 80-95% VO2max, interspersed by 1 min active rest at 50-60% VO2max) and male and female long bouts HIIE (MHIIEL and FMHIIEL respectively, 4×4 min work at of 80-95% VO2max, interspersed by 4 min active rest at 50-60% VO2max). Rats were sacrificed immediately after exercise and the heart muscle was isolated to measure the telomerase enzyme activity, TOS and TAC levels. Statistical analysis was performed by two-way ANOVA and Tukey post hoc test (P<0.05).
Results: The level of telomerase enzyme activity and TOS level didn’t change after HIIE in male and female rats (P=0.52 and 0.69 respectively), although the 9 and 11.5 percentage increase in telomerase enzyme activity were observed in FMHIIESh and FMHIIEL than control group. The level of TAC in response to short and long high intensity interval exercise bouts in male and females rats decreased significantly (P=0.01). Furthermore, there was a significant difference between male and female rats in response to two different HIIE (P=0.005); such that in the HIIESh groups, there was only a significant decrease in male rats (P=0.04). Also, in the HIIEL groups, a significant decrease was observed in both male and female rats (P=0.03 and 0.04 respectively).
Discussion: Acute exposure to short and long high intensity interval exercise bouts will not result a significant change in some indicators of biological aging. However, due to the differences in biology between genders, more studies provide important evidence regarding the roles of high intensity interval exercise with different time of bouts that play in preventing aging.
Methodology: 40 male Wistar rats (aged 12 weeks) were randomly assigned to six groups: male and female control (two groups), male and female short bouts HIIE (MHIIESh and FMHIIESh respectively, 16×1 min work at of 80-95% VO2max, interspersed by 1 min active rest at 50-60% VO2max) and male and female long bouts HIIE (MHIIEL and FMHIIEL respectively, 4×4 min work at of 80-95% VO2max, interspersed by 4 min active rest at 50-60% VO2max). Rats were sacrificed immediately after exercise and the heart muscle was isolated to measure the telomerase enzyme activity, TOS and TAC levels. Statistical analysis was performed by two-way ANOVA and Tukey post hoc test (P<0.05).
Results: The level of telomerase enzyme activity and TOS level didn’t change after HIIE in male and female rats (P=0.52 and 0.69 respectively), although the 9 and 11.5 percentage increase in telomerase enzyme activity were observed in FMHIIESh and FMHIIEL than control group. The level of TAC in response to short and long high intensity interval exercise bouts in male and females rats decreased significantly (P=0.01). Furthermore, there was a significant difference between male and female rats in response to two different HIIE (P=0.005); such that in the HIIESh groups, there was only a significant decrease in male rats (P=0.04). Also, in the HIIEL groups, a significant decrease was observed in both male and female rats (P=0.03 and 0.04 respectively).
Discussion: Acute exposure to short and long high intensity interval exercise bouts will not result a significant change in some indicators of biological aging. However, due to the differences in biology between genders, more studies provide important evidence regarding the roles of high intensity interval exercise with different time of bouts that play in preventing aging.
Keywords
High intensity interval exercise; Telomerase enzyme activity; Total oxidant/antioxidant status; Male; Female
Subjects