The effect of 6 weeks of high intensity interval training preconditioning on clinical outcome and demyelination in cerebellum tissue of female mice with multiple sclerosis in EAE model
Poster Presentation XML
Paper ID : 1674-11THCONF
1دانشکده تربیت بدنی و علوم ورزشی دانشگاه شهید بهشتی
2دانشگاه علوم پزشکی ایران-دانشکده پیراپزشکی
3دانشگاه خوارزمی-دانشکده تربیت بدنی وعلوم ورزشی
4دانشگاه علوم بهزیستی و توانبخشی
Introduction: Multiple sclerosis (MS) is a common disorder of the central nervous system characterized by inflammation, demyelination and axonal degeneration. To further understand molecular regulation during the acute phase of the disease, mouse models of experimental autoimmune encephalomyelitis (EAE) have been used. The impact of exercise on disease progression in multiple sclerosis (MS) is unclear. In this study, we investigated the effects of preconditioning exercise on the clinical and pathological progression of EAE.
Methodology: Twenty Female C57BL/6 mice (6-8 weeks old) were maintained on a 12:12 h light: dark cycle and were provided with food and water ad libitum. The animals were randomly assigned to either an exercised (Ex, n=10) or unexercised (UEx (EAE)), n=10) group and all of them were induced for EAE. Mice in EX group were run 5 day /week on a motorized treadmill for 6 weeks, at a velocity corresponding to 80% at first week to 110% of the maximal running velocity previously determined by an incremental exercise test, at Sixth session. All animals were familiarized with running on a motor-driven treadmill (5 days, 8min/day, 10m/min). Mice in EX and UEx group were anesthetized in 23 day post induction EAE (high score). Demyelination pathological analysis of exercised and unexercised EAE mice were measured by LFB.
Results: Mice in the Ex group had an attenuated clinical score relative to UEx mice throughout the study. Ex mice also had a significant reduction in myelin damage.
Discussion: Our study demonstrates for the first time that a prolonged and forced preconditioning protocol of exercise improves clinical outcome and attenuates pathological hallmarks of EAE at chronic disease.