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The Effect of high intensity interval training on myocardial ischemia reperfusion injury in male Wistar rats
Poster Presentation
Paper ID : 1705-11THCONF
Authors
1عضو هیات علمی دانشگاه آزاد اسلامی واحد مریوان
2Assistant Professor of sport Physiology, Islamic Azad University, Sanadaj branch.
3Master's student in of Sport Physiology, Islamic Azad University, Marivan Branch.
4Assistant Professor of sport Physiology, Islamic Azad University, Bandar Abbas branch.
Abstract
Introduction: Ischemic preconditioning provides protective effects on ischemia - reperfusion Injury. The aim of the present study was to study the protective effect of high intensity interval training on stress oxidative and myocardial infarction–induced ischemia - reperfusion injury in male Wistar rats.
Methodology: 20 male Wistar rats were randomly assigned in control (Con; n=10) and high intensity interval training (HIIT; n=10). Exercise training was done for 8 weeks, 5 days per week and lasted 40 min per session. Intense interval training consists of 10 intervals of the running with an intensity of 90% of maximal oxygen consumption (VO2max) for 2 min and 1 min of running with 50% VO2max. 72 hr after last training session, animals were underwent surgery, and coronary occlusion was achieved by blocking aorta; for 30 min followed by a 120-min period of reperfusion. Glutathione peroxidase (GPX), Glutathione (GSH), Catalase (CAT), Myeloperoxidase (MPO), Malondialdehyde (MDA) and Myocardial infarct size were measured.
Results: Results showed that there was no significant difference between HIIT and Control in GPX and MPO activity following ischemia-reperfusion (p˃0.05). However, CAT activity (p=0.004) and GSH content significantly were higher in HIIT than control. Also, MDA concentration was greater in HIIT than control (p=0.006). While, myocardial infarct size significantly was lesser in HIIT than control (p=0.004).
Discussion: Finally, it can be said that 8 weeks of high intensity interval training can prevent myocardial infarction following ischemia- reperfusion by preconditioning via improving some antioxidant factors, and could been considered as important precautionary method in myocardial infarction events.
Methodology: 20 male Wistar rats were randomly assigned in control (Con; n=10) and high intensity interval training (HIIT; n=10). Exercise training was done for 8 weeks, 5 days per week and lasted 40 min per session. Intense interval training consists of 10 intervals of the running with an intensity of 90% of maximal oxygen consumption (VO2max) for 2 min and 1 min of running with 50% VO2max. 72 hr after last training session, animals were underwent surgery, and coronary occlusion was achieved by blocking aorta; for 30 min followed by a 120-min period of reperfusion. Glutathione peroxidase (GPX), Glutathione (GSH), Catalase (CAT), Myeloperoxidase (MPO), Malondialdehyde (MDA) and Myocardial infarct size were measured.
Results: Results showed that there was no significant difference between HIIT and Control in GPX and MPO activity following ischemia-reperfusion (p˃0.05). However, CAT activity (p=0.004) and GSH content significantly were higher in HIIT than control. Also, MDA concentration was greater in HIIT than control (p=0.006). While, myocardial infarct size significantly was lesser in HIIT than control (p=0.004).
Discussion: Finally, it can be said that 8 weeks of high intensity interval training can prevent myocardial infarction following ischemia- reperfusion by preconditioning via improving some antioxidant factors, and could been considered as important precautionary method in myocardial infarction events.
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