The impact of a session of eccentric endurance exercise as Adjuvant in HSV-gD1 DNA vaccine on the ratio of INF-Ƴ to IL-4 cytokines in spleen tissue of Balb/c Mice
Poster Presentation XML
Authors
1tarbiat modares university
2Department of Physical Education and Sports Science, University of Tarbiat Modares, Tehran, Iran.
3Department of Immunology, University of Tarbiat Modares, Tehran, Iran.
4Department of Virology, University of Tarbiat Modares, Tehran, Iran
5Department of Physical Education and Sports Science, University of Tarbiat Modares, Tehran, Iran
Abstract
Introduction: The activity of immune cells and the releasing of inflammatory mediators following exercise-induced muscle damage may result in a pro-inflammatory environment that increases immune responses to the vaccine by releasing risk signals or activating DCs. According to this hypothesis, the purpose of this study was to investigate the impact of a session of eccentric endurance exercise as adjuvant in HSV-gD1 DNA vaccine on the ratio of INF-γ to IL-4 cytokines in spleen tissue of Balb/c Mice.
Methodology: Balb/c mice (6 to 8 weeks) were randomly assigned into six groups, 1- eccentric endurance exercise, 2- Vaccine, 3-PCDNA, 4- control, 5- eccentric endurance exercise + Vaccine and 6- eccentric endurance exercise + PCDNA. The exercise protocol included one session of eccentric endurance exercise on the animal treadmill. 48 hours before the eccentric endurance exercise, HSV-gD1 DNA vaccine was injected into the tibialis muscle of mice. 12 days after the second vaccine, the ratio of INF-γ and IL-4 cytokines were evaluated by ELISA method in spleen tissue. One-way ANOVA was used to analyze the data.
Results: The results showed that a session of Eccentric endurance exercise significantly increased the ratio of INF-γ and IL-4 cytokines in the HSV-gD1 vaccine model. Also, there is a significant difference between this group and other groups, especially the vaccine group.
Discussion: Generally, a session of eccentric endurance exercise as Adjuvant in HSV-gD1 DNA vaccine, can increase the ratio of INF-Ƴ to IL-4 cytokines in spleen tissue of Balb/c Mice.
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